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Anti-Human TAF3 Polyclonal Antibody (HD303014)

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Overview
Catalog No.HD303014
Description
Anti-Human TAF3 Polyclonal Antibody (HD303014) is a rabbit polyclonal antibody detecting TAF3. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TAF3 (Cys2-Phe99).
Target 140 kDa TATA box-binding protein-associated factor, TAF(II)140, TAF140, TAF3, TAFII-140, TAFII140, TBP-associated factor 3, Transcription initiation factor TFIID 140 kDa subunit, Transcription initiation factor TFIID subunit 3
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q5VWG9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Transcription initiation factor TFIID subunit 3 (TAF3) is a ~103 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TAF3 forms the TFIID-A module together with TAF5 and TBP.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Gangloff, YG. et al. (2001) Molecular and cellular biology 21, 5109-21. PMID: 11438666
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human TAF3 Polyclonal Antibody

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