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Anti-Human TBP Polyclonal Antibody (HB847014)

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Overview
Catalog No.HB847014
Description
Anti-Human TBP Polyclonal Antibody (HB847014) is a rabbit polyclonal antibody detecting TBP in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TBP (Ala155-Phe335).
Target TF2D, TATA-binding factor, GTF2D1, Transcription initiation factor TFIID TBP subunit, TFIID, TBP, TATA sequence-binding protein, TATA-box factor, TATA-box-binding protein
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P20226, P29037
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

TATA-box-binding protein (TBP) is a ~37 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TBP forms the TFIID-A module together with TAF3 and TAF5.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Kao, CC. et al. (1990) Science (New York, N.Y.) 248, 1646-50. PMID: 2194289
3. Peterson, MG. et al. (1990) Science (New York, N.Y.) 248, 1625-30. PMID: 2363050
4. Hoffman, A. et al. (1990) Nature 346, 387-90. PMID: 2374612
5. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
6. Louder, RK. et al. (2016) Nature 531, 604-9. PMID: 27007846
Note For research use only.
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Formula
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