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Anti-Human TBX5 Polyclonal Antibody (HP677014)

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Overview
Catalog No.HP677014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TBX5 (Gln43-Lys253).
Target T-box protein 5, T-box transcription factor TBX5, TBX5
Purification Purified by antigen affinity column.
Accession Q99593
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

T-box transcription factor TBX5 is a ~57 kDa protein. DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. Binds to the core DNA motif of NPPA promoter.

1. Zhang, XL. et al. (2015) Biochemical and biophysical research communications 459, 166-71. PMID: 25725155
2. Zhou, W. et al. (2015) International journal of molecular medicine 36, 282-8. PMID: 25963046
3. Wang, ZC. et al. (2016) International journal of medical sciences 13, 60-7. PMID: 26917986
4. Guo, DF. et al. (2016) Molecular medicine reports 13, 4349-56. PMID: 27035640
5. Ghosh, TK. et al. (2018) Journal of molecular and cellular cardiology 114, 185-198. PMID: 29174768
6. Li, QY. et al. (1997) Nature genetics 15, 21-9. PMID: 8988164
7. Pradhan, L. et al. (2016) Biochemistry 55, 1702-10. PMID: 26926761
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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