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Anti-Human TBX6 Polyclonal Antibody (HF903014)

Anti-Human TBX6 Polyclonal Antibody
Anti-Human TBX6 Polyclonal Antibody
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Overview
Catalog No.HF903014
Description
Anti-Human TBX6 Polyclonal Antibody (HF903014) is a rabbit polyclonal antibody detecting TBX6 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TBX6 (Gly90-Lys289).
Target T-box transcription factor TBX6, T-box protein 6, TBX6
Purification Purified by antigen affinity column.
Accession O95947
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

T-box transcription factor TBX6 is a ~47 kDa protein. T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. Acts in part by down-regulating, a specific enhancer (N1) of SOX2, to inhibit neural development. Seems to play also an essential role in left/right axis determination and acts through effects on Notch signaling around the node as well as through an effect on the morphology and motility of the nodal cilia.

Note For research use only.
Images
  • Anti-Human TBX6 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TBX6 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Raji cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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