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Anti-Human TEX14 Polyclonal Antibody (HP432014)

Anti-Human TEX14 Polyclonal Antibody
Anti-Human TEX14 Polyclonal Antibody
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Overview
Catalog No.HP432014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TEX14 (Cys24-Arg185).
Target Sugen kinase 307, Protein kinase-like protein SgK307, SGK307, TEX14, Inactive serine/threonine-protein kinase TEX14, Testis-expressed sequence 14 protein, Testis-expressed sequence 14
Purification Purified by antigen affinity column.
Accession Q8IWB6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Inactive serine/threonine-protein kinase TEX14 is a ~167 kDa protein. Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro.

Note For research use only.
Images
  • Anti-Human TEX14 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Human TEX14 polyclonal antibody at 1ug/mL on recombinant Human TEX14 (Catalog No. HP432012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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