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Anti-Human TFCP2 Polyclonal Antibody (HC653014)

Anti-Human TFCP2 Polyclonal Antibody
Anti-Human TFCP2 Polyclonal Antibody
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Overview
Catalog No.HC653014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TFCP2 (Gln68-Gln266).
Target SEF, LSF, SAA3 enhancer factor, TFCP2, Transcription factor LSF, Alpha-globin transcription factor CP2
Purification Purified by antigen affinity column.
Accession Q12800
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Alpha-globin transcription factor CP2 (TFCP2) is a ~57 kDa protein. Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1. Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter.

Note For research use only.
Images
  • Anti-Human TFCP2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Human TFCP2 polyclonal antibody at 1ug/mL on recombinant Human TFCP2 (Catalog No. HC653012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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