Anti-Human THOC7 Polyclonal Antibody (HA527014)

Name: Anti-Human THOC7 Polyclonal Antibody
Brand: abinScience
SKU: HA527014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HA527014

Description

Anti-Human THOC7 Polyclonal Antibody (HA527014) is a rabbit polyclonal antibody detecting THOC7 in ELISA, IHC, WB. Suitable for Xenopus tropicalis, Xenopus laevis, Bovine, and Mus musculus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human THOC7 (Met1-Pro204).

Target

THOC7

Purification

Purified by antigen affinity column.

Accession

Q6I9Y2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

THO complex subunit 7 homolog, Functional spliceosome-associated protein 24, fSAP24, Ngg1-interacting factor 3-like protein 1-binding protein 1, NIF3L1-binding protein 1, hTREX30, THOC7, NIF3L1BP1

Background

THO complex subunit 7 (THOC7) is a ~23 kDa protein. Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. Required for efficient export of polyadenylated RNA. Plays a key structural role in the oligomerization of the THO-DDX39B complex. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway.

1. Guo, S. et al. (2005) Cancer research 65, 3011-6. PMID: 15833825

2. Masuda, S. et al. (2005) Genes & development 19, 1512-7. PMID: 15998806

3. Cheng, H. et al. (2006) Cell 127, 1389-400. PMID: 17190602

4. Chi, B. et al. (2013) Nucleic acids research 41, 1294-306. PMID: 23222130

5. Pühringer, T. et al. (2020) eLife 9. PMID: 33191911

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-THOC7 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse skin lysate

References

Datasheet

Anti-Human THOC7 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human THOC7 Polyclonal Antibody (HA527014)

Overview

Images

References

Datasheet

SDS

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