Anti-Human TIF1/TRIM24 IP Recombinant Antibody (SAA0265) (HT332013)

Name: Anti-Human TIF1/TRIM24 IP Recombinant Antibody (SAA0265)
Brand: abinScience
SKU: HT332013
Price: 191 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT332013

Species reactivity

Human

Applications

ELISA, IF, IP

Host species

Mouse

Isotype

IgG2a

Clone ID

SAA0265

Clonality

Monoclonal

Target

RING-type E3 ubiquitin transferase TIF1-alpha, TIF1-alpha, RING finger protein 82, Transcription intermediary factor 1-alpha, RNF82, Tripartite motif-containing protein 24, E3 ubiquitin-protein ligase TRIM24, TIF1, TIF1A, TRIM24

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O15164

RRID

Anti-Human TIF1/TRIM24 IP Antibody (SAA0265) (abinScience Cat# HT332013, RRID:AB_3729799)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Transcription intermediary factor 1-alpha (TIF1/TRIM24) is a ~116 kDa protein. Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation.

1. Jain, AK. et al. (2014) Molecular and cellular biology 34, 2695-709. PMID: 24820418

2. Zhu, Q. et al. (2020) The Journal of experimental medicine 217. PMID: 32324863

3. Hang, Y. et al. (2021) Cell biology international 45, 1561-1570. PMID: 33724611

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human TIF1/TRIM24 IP Antibody (SAA0265)

References

Datasheet

Anti-Human TIF1/TRIM24 IP Recombinant Antibody (SAA0265).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TIF1/TRIM24 IP Recombinant Antibody (SAA0265) (HT332013)

Overview

Images

References

Datasheet

SDS

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