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Anti-Human TIMM50 Polyclonal Antibody (HS707014)

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Overview
Catalog No.HS707014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TIMM50 (Gln145-Pro353).
Target TIM50, TIMM50, Mitochondrial import inner membrane translocase subunit TIM50
Purification Purified by antigen affinity column.
Accession Q3ZCQ8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mitochondrial import inner membrane translocase subunit TIM50 (TIMM50) is a ~39 kDa protein. Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.

1. Reyes, A. et al. (2018) EMBO molecular medicine 10. PMID: 30190335
2. Crameri, JJ. et al. (2024) Molecular and cellular biology 44, 226-244. PMID: 38828998
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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