Anti-Human TJP3 Polyclonal Antibody (HC970014)

Name: Anti-Human TJP3 Polyclonal Antibody
Brand: abinScience
SKU: HC970014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HC970014

Description

Anti-Human TJP3 Polyclonal Antibody (HC970014) is a rabbit polyclonal antibody detecting TJP3. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TJP3 (Ser378-Asp776).

Target

TJP3, Tight junction protein 3, Tight junction protein ZO-3, ZO3, Zona occludens protein 3, Zonula occludens protein 3

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O95049

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Tight junction protein ZO-3 (TJP3) is a ~101 kDa protein. TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PATJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions. Promotes cell-cycle progression through the sequestration of cyclin D1 (CCND1) at tight junctions during mitosis which prevents CCND1 degradation during M-phase and enables S-phase transition. With TJP1 and TJP2, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus.

1. Michel, D. et al. (2005) Journal of cell science 118, 4049-57. PMID: 16129888

2. Capaldo, CT. et al. (2011) Molecular biology of the cell 22, 1677-85. PMID: 21411630

Note

For research use only

Images

References

Datasheet

Anti-Human TJP3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human TJP3 Polyclonal Antibody (HC970014)

Overview

Images

References

Datasheet

SDS

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