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Anti-Human TMEM165 Polyclonal Antibody (HP219014)

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Overview
Catalog No.HP219014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TMEM165 (Gly34-Thr89).
Target TPARL, TMEM165, Transmembrane protein PT27, Transmembrane protein TPARL, Transmembrane protein 165
Purification Purified by antigen affinity column.
Accession Q9HC07
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Putative divalent cation/proton antiporter TMEM165 is a ~34 kDa protein. Putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. Mediates the reversible transport of calcium or manganese to the Golgi lumen driven by the proton gradient and possibly the membrane potential generated by V-ATPase. Provides calcium or manganese cofactors to resident Golgi enzymes and contributes to the maintenance of an acidic luminal Golgi pH required for proper functioning of the secretory pathway. Promotes Ca(2+) storage within the Golgi lumen of the mammary epithelial cells to be then secreted into milk. The transport mechanism and stoichiometry remains to be elucidated.

1. Foulquier, F. et al. (2012) American journal of human genetics 91, 15-26. PMID: 22683087
2. Demaegd, D. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 6859-64. PMID: 23569283
3. Potelle, S. et al. (2016) Human molecular genetics 25, 1489-500. PMID: 27008884
4. Stribny, J. et al. (2020) The Journal of biological chemistry 295, 3865-3874. PMID: 32047108
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human TMEM165 Polyclonal Antibody

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