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Anti-Human TPI1 Polyclonal Antibody (HX910014)

Anti-Human TPI1 Polyclonal Antibody
Anti-Human TPI1 Polyclonal Antibody
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Overview
Catalog No.HX910014
Description
Anti-Human TPI1 Polyclonal Antibody (HX910014) is a rabbit polyclonal antibody detecting TPI1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Dog.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TPI1 (Met1-Gln249).
Target TIM, Triose-phosphate isomerase, TPI, Methylglyoxal synthase, Triosephosphate isomerase, TPI1
Purification Purified by antigen affinity column.
Accession P60174
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Triosephosphate isomerase (TPI1) is a ~26 kDa protein. Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.

Note For research use only.
Images
  • Anti-Human TPI1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TPI1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: 293T cell lysate
    Lane 4: U87 cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: K562 cell lysate
    Lane 7: Hela cell lysate
    Lane 8: Mouse brain lysate
    Lane 9: Mouse liver lysate
    Lane 10: Mouse kidney lysate
    Lane11: Mouse skeletal muscle lysate
    Lane12: Rat brain lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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