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Anti-Human TRAF3IP2 Polyclonal Antibody (HT250014)

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Overview
Catalog No.HT250014
Description
Anti-Human TRAF3IP2 Polyclonal Antibody (HT250014) is a rabbit polyclonal antibody detecting TRAF3IP2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TRAF3IP2 (Ser403-Arg554).
Target E3 ubiquitin ligase TRAF3IP2, 2.3.2.27, Adapter protein CIKS, Connection to IKK and SAPK/JNK, E3 ubiquitin-protein ligase CIKS, Nuclear factor NF-kappa-B activator 1, ACT1, TRAF3-interacting protein 2, TRAF3IP2, C6orf2, C6orf4, C6orf5, C6orf6
Purification Purified by antigen affinity column.
Accession O43734
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

E3 ubiquitin ligase TRAF3IP2 is a ~64 kDa protein. E3 ubiquitin ligase that catalyzes 'Lys-63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling. Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein. Essential adapter molecule in IL17A-mediated signaling. Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys-63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways.

1. Liu, C. et al. (2009) Science signaling 2, ra63. PMID: 19825828
2. Boisson, B. et al. (2013) Immunity 39, 676-86. PMID: 24120361
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human TRAF3IP2 Polyclonal Antibody

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