Anti-Human TRBC1 Recombinant Antibody (Jovi-1) (HP894107)

Name: Anti-Human TRBC1 Recombinant Antibody (Jovi-1)
Brand: abinScience
SKU: HP894107
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HP894107
Species reactivity	Human
Applications	ELISA, FCM, IF
Host species	Mouse
Isotype	IgG2a, kappa
Clone ID	Jovi-1
Clonality	Monoclonal
Target	TRBC1, T cell receptor beta constant 1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01850
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	T cell receptor beta constant 1 (TRBC1) is a ~19 kDa protein. Constant region of T cell receptor (TR) beta chain. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. 1. Lefranc, MP. (2014) Frontiers in immunology 5, 22. PMID: 24600447 2. Rossjohn, J. et al. (2015) Annual review of immunology 33, 169-200. PMID: 25493333 3. Brownlie, RJ. et al. (2013) Nature reviews. Immunology 13, 257-69. PMID: 23524462 4. Bäckström, BT. et al. (1997) The Journal of experimental medicine 186, 1933-8. PMID: 9382891 5. Nikolich-Zugich, J. et al. (2004) Nature reviews. Immunology 4, 123-32. PMID: 15040585
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human TRBC1 Antibody (Jovi-1).
Flow Cytometry

Flow-cytometry using anti-human TRBC1 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human TRBC1 antibody monoclonal antibody (Catalog # HP894107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human TRBC1 Recombinant Antibody (Jovi-1).pdf

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Anti-Human TRBC1 Recombinant Antibody (Jovi-1) (HP894107)

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