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Anti-Human TRDMT1 Polyclonal Antibody (HT258014)

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Overview
Catalog No.HT258014
Description
Anti-Human TRDMT1 Polyclonal Antibody (HT258014) is a rabbit polyclonal antibody detecting TRDMT1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TRDMT1 (Met1-Gln170).
Target tRNA (cytosine(38)-C(5))-methyltransferase, 2.1.1.204, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, M.HsaIIP, PuMet, TRDMT1, DNMT2
Purification Purified by antigen affinity column.
Accession O14717
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

tRNA (cytosine(38)-C(5))-methyltransferase (TRDMT1) is a ~44 kDa protein. Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Has higher activity on tRNA(Asp) modified with queuosine at position 34.

1. Goll, MG. et al. (2006) Science (New York, N.Y.) 311, 395-8. PMID: 16424344
2. Tuorto, F. et al. (2018) The EMBO journal 37. PMID: 30093495
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human TRDMT1 Polyclonal Antibody

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