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Anti-Human TRIM15 Polyclonal Antibody (HP976014)

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Overview
Catalog No.HP976014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TRIM15 (Glu80-Pro275).
Target ZNF178, Tripartite motif-containing protein 15, TRIM15, ZNFB7, Zinc finger protein 178, RNF93, RING finger protein 93, Zinc finger protein B7
Purification Purified by antigen affinity column.
Accession Q9C019
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

E3 ubiquitin-protein ligase TRIM15 is a ~52 kDa protein. E3 ubiquitin ligase that plays a role in several processes including innate antiviral immnity, cell migration and chemotaxis. Acts as a 'Lys-63'-specific ubiquitin ligase for MAPK1/ERK2 and MAPK3/ERK1, promoting their activation by facilitating their interaction with MAP2K1 and MAP2K2. Also plays a role in cell migration and chemotaxis by acting as a stable focal adhesion component upon recruitment by multi-adapter protein paxillin/PXN. Functions in the RIGI-mediated interferon induction pathway upstream or at the level of MAVS. Inhibits NF-kappa-B activation by turnover of 'Lys-63'-linked ubiquitination of MAP3K7/TAK1.

1. Uchil, PD. et al. (2013) Journal of virology 87, 257-72. PMID: 23077300
2. Zhang, J. et al. (2021) Journal of molecular histology 52, 839-848. PMID: 34142270
3. Zhu, G. et al. (2021) Nature cell biology 23, 978-991. PMID: 34497368
4. Uchil, PD. et al. (2014) Journal of cell science 127, 3928-42. PMID: 25015296
5. Roy, M. et al. (2022) Cellular signalling 91, 110210. PMID: 34871740
Note For research use only.
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Formula
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Anti-Human TRIM15 Polyclonal Antibody

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