Anti-Human TRIM22 Polyclonal Antibody (HC134014)

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Overview

Catalog No.

HC134014

Description

Anti-Human TRIM22 Polyclonal Antibody (HC134014) is a rabbit polyclonal antibody detecting TRIM22. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TRIM22 (Ser317-Ser498).

Target

50 kDa-stimulated trans-acting factor, E3 ubiquitin-protein ligase TRIM22, EC:2.3.2.27, RING finger protein 94, RING-type E3 ubiquitin transferase TRIM22, RNF94, STAF50, Staf-50, TRIM22, Tripartite motif-containing protein 22

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q8IYM9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

E3 ubiquitin-protein ligase TRIM22 is a ~56 kDa protein. Interferon-induced E3 ubiquitin ligase that plays important roles in innate and adaptive immunity. Restricts the replication of many viruses including HIV-1, encephalomyocarditis virus (EMCV), hepatitis B virus (HBV), hepatitis C virus (HCV) or Zika virus (ZIKV). Mechanistically, negatively regulates HCV replication by promoting ubiquitination and subsequent degradation of viral NS5A. Also acts by promoting the degradation of Zika virus NS1 and NS3 proteins through proteasomal degradation. Acts as a suppressor of basal HIV-1 LTR-driven transcription by preventing Sp1 binding to the HIV-1 promoter.

1. Yang, C. et al. (2016) Cellular & molecular immunology 13, 94-102. PMID: 25683609

2. Inoue, A. et al. (2022) Biochimica et biophysica acta. Molecular cell research 1869, 119318. PMID: 35777501

3. Zu, S. et al. (2022) Cell & bioscience 12, 139. PMID: 36042495

4. Turrini, F. et al. (2015) Retrovirology 12, 104. PMID: 26683615

5. Fei, X. et al. (2022) Molecular therapy oncolytics 26, 413-428. PMID: 36159777

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-TRIM22 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: K562 cell lysate
Lane 3: HL-60 cell lysate
Lane 4: Mouse thymus lysate
Lane 5: Mouse lung lysate

References

Datasheet

Anti-Human TRIM22 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TRIM22 Polyclonal Antibody (HC134014)

Overview

Images

References

Datasheet

SDS

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