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Anti-Human TRIM72 Polyclonal Antibody (HD364014)

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Overview
Catalog No.HD364014
Description
Anti-Human TRIM72 Polyclonal Antibody (HD364014) is a rabbit polyclonal antibody detecting TRIM72. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TRIM72 (Leu288-Ala477).
Target EC:2.3.2.27, MG53, Mg53, Mitsugumin-53, TRIM72, Tripartite motif-containing protein 72
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6ZMU5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Tripartite motif-containing protein 72 (TRIM72) is a ~52 kDa protein. Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process.

1. Ma, Y. et al. (2023) Nature communications 14, 1555. PMID: 36944613
Note For research use only
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Formula
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Anti-Human TRIM72 Polyclonal Antibody

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