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Anti-Human TRNAU1AP Polyclonal Antibody (HA201014)

Anti-Human TRNAU1AP Polyclonal Antibody
Anti-Human TRNAU1AP Polyclonal Antibody
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Overview
Catalog No.HA201014
Description
Anti-Human TRNAU1AP Polyclonal Antibody (HA201014) is a rabbit polyclonal antibody detecting TRNAU1AP in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TRNAU1AP (Thr361-Gln657).
Target SECp43, tRNA selenocysteine 1-associated protein 1, TRSPAP1, tRNA selenocysteine-associated protein 1, TRNAU1AP, SECP43
Purification Purified by antigen affinity column.
Accession Q9NX07
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

tRNA selenocysteine 1-associated protein 1 (TRNAU1AP) is a ~32 kDa protein. Involved in the early steps of selenocysteine biosynthesis and tRNA(Sec) charging to the later steps resulting in the cotranslational incorporation of selenocysteine into selenoproteins. Stabilizes the SECISBP2, EEFSEC and tRNA(Sec) complex. May be involved in the methylation of tRNA(Sec). Enhances efficiency of selenoproteins synthesis.

1. Small-Howard, A. et al. (2006) Molecular and cellular biology 26, 2337-46. PMID: 16508009
Note For research use only.
Images
  • Anti-Human TRNAU1AP Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PTPRR polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: HCT116 cell lysate
    Lane 3: SKOV3 cell lysate
    Lane 4: U87 cell lysate
    Lane 5: HT-29 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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