Anti-Human TUBB/Beta Tubulin Recombinant Antibody (SAA2334) (HY374013)

Name: Anti-Human TUBB/Beta Tubulin Recombinant Antibody (SAA2334)
Brand: abinScience
SKU: HY374013
Price: 126 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY374013

Species reactivity

Human

Applications

ELISA, IF, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA2334

Clonality

Monoclonal

Target

Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5, β tubulin

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P07437

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Tubulin beta chain (TUBB) is a ~49 kDa protein. Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Note

For research use only

Images

SDS-PAGE

SDS-PAGE for Anti-Human TUBB/Beta Tubulin Antibody (SAA2334)
Western blot

Western blot analysis was performed using anti-beta Tubulin monoclonal antibody at 1ug/mL on various samples.
Lane 1: U251 cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A431 cell lysate
Lane 5: NIH3T3 cell lysate
Lane 6: Jurkat cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: Lncap cell lysate
Lane 9: Mouse brain lysate
Lane 10: Rat brain lysate