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Anti-Human TUBG1 Polyclonal Antibody (HB833014)

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Anti-Human TUBG1 Polyclonal Antibody
Anti-Human TUBG1 Polyclonal Antibody
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Overview
Catalog No.HB833014
Description
Anti-Human TUBG1 Polyclonal Antibody (HB833014) is a rabbit polyclonal antibody detecting TUBG1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human TUBG1 (Met1-Gln451).
Target Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1, TUBG1, TUBG
Purification Purified by antigen affinity column.
Accession P23258
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Tubulin gamma-1 chain (TUBG1) is a ~51 kDa protein. Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.

1. Brito, C. et al. (2024) Science (New York, N.Y.) 383, 870-876. PMID: 38305685
2. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661
3. Serna, M. et al. (2024) Developmental cell 59, 3175-3188.e8. PMID: 39321809
Note For research use only.
Images
  • Anti-Human TUBG1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TUBG1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: K562 cell lysate
    Lane 4: Hela cell lysate
    Lane 5: Jurkat cell lysate
    Lane 6: A431 cell lysate
    Lane 7: C2C12 cell lysate
    Lane 8: U2OS cell lysate
    Lane 9: A549 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human TUBG1 Polyclonal Antibody

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