Anti-Human TUFM Polyclonal Antibody (HW446014)

Name: Anti-Human TUFM Polyclonal Antibody
Brand: abinScience
SKU: HW446014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW446014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TUFM (Asp67-Gly452).

Target

P43, Elongation factor Tu, mitochondrial, TUFM, EF-Tu

Purification

Purified by antigen affinity column.

Accession

P49411

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Elongation factor Tu, mitochondrial (TUFM) is a ~49 kDa protein. GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Participates in mitochondrial translation. Also plays a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.

1. Lei, Y. et al. (2012) Immunity 36, 933-46. PMID: 22749352

2. Ding, B. et al. (2017) Cell host & microbe 21, 538-547.e4. PMID: 28407488

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-TUFM polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: Mouse brain lysate
Lane 4: Mouse kidney lysate
Lane 5: Mouse liver lysate
Lane 6: Rat brain lysate
Lane 7: Rat kidney lysate
Lane 8: Rat liver lysate