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Anti-Human TYR Polyclonal Antibody (HB200014)

Anti-Human TYR Polyclonal Antibody
Anti-Human TYR Polyclonal Antibody
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Overview
Catalog No.HB200014
Description
Anti-Human TYR Polyclonal Antibody (HB200014) is a rabbit polyclonal antibody detecting TYR in ELISA, IHC, WB. Suitable for Mus musculus, Human, Felis catus, and Bovine.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human TYR (His19-Ser476).
Target TYR
Purification Purified by antigen affinity column.
Accession P14679
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesTyrosinase, 1.14.18.1, LB24-AB, Monophenol monooxygenase, SK29-AB, Tumor rejection antigen AB, TYR
Background

Tyrosinase is a ~60 kDa protein. This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.

1. Lai, X. et al. (2017) Angewandte Chemie (International ed. in English) 56, 9812-9815. PMID: 28661582
Note For research use only.
Images
  • Anti-Human TYR Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TYR polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Rat lung lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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