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Anti-Human U2AF2 Polyclonal Antibody (HB779014)

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Anti-Human U2AF2 Polyclonal Antibody
Anti-Human U2AF2 Polyclonal Antibody
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Overview
Catalog No.HB779014
Description
Anti-Human U2AF2 Polyclonal Antibody (HB779014) is a rabbit polyclonal antibody detecting U2AF2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human U2AF2 (Pro138-Gly338).
Target Splicing factor U2AF 65 kDa subunit, hU2AF65, hU2AF(65), U2 auxiliary factor 65 kDa subunit, U2AF65, U2AF2, U2 snRNP auxiliary factor large subunit
Purification Purified by antigen affinity column.
Accession P26368
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Splicing factor U2AF 65 kDa subunit (U2AF2) is a ~53 kDa protein. Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly.

1. Millevoi, S. et al. (2006) The EMBO journal 25, 4854-64. PMID: 17024186
2. David, CJ. et al. (2011) Genes & development 25, 972-83. PMID: 21536736
Note For research use only.
Images
  • Anti-Human U2AF2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-U2AF2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K562 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human U2AF2 Polyclonal Antibody

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