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Anti-Human UBE2K Polyclonal Antibody (HX857014)

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Overview
Catalog No.HX857014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human UBE2K (Met1-Asn200).
Target Ubiquitin-conjugating enzyme E2(25K), Ubiquitin-protein ligase, UBE2K, HIP-2, E2 ubiquitin-conjugating enzyme K, Huntingtin-interacting protein 2, Ubiquitin-conjugating enzyme E2-25K, Ubiquitin-conjugating enzyme E2 K, Ubiquitin carrier protein, Ubiquitin-conjugating enzyme E2-25 kDa, HIP2, LIG
Purification Purified by antigen affinity column.
Accession P61086
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ubiquitin-conjugating enzyme E2 K (UBE2K) is a ~22 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin.

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human UBE2K Polyclonal Antibody

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