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Anti-Human UBE4B Polyclonal Antibody (HD151014)

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Anti-Human UBE4B Polyclonal Antibody
Anti-Human UBE4B Polyclonal Antibody
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Overview
Catalog No.HD151014
Description
Anti-Human UBE4B Polyclonal Antibody (HD151014) is a rabbit polyclonal antibody detecting UBE4B. Suitable for Human and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human UBE4B (Leu961-His1302).
Target EC:2.3.2.27, HDNB1, Homozygously deleted in neuroblastoma 1, KIAA0684, RING-type E3 ubiquitin transferase E4 B, UBE4B, UFD2, Ubiquitin conjugation factor E4 B, Ubiquitin fusion degradation protein 2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O95155
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Ubiquitin conjugation factor E4 B (UBE4B) is a ~146 kDa protein. Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation.

1. Janiesch, PC. et al. (2007) Nature cell biology 9, 379-90. PMID: 17369820
Note For research use only
Images
  • Anti-Human UBE4B Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-UBE4B polyclonal antibody at 1ug/mL on various samples.
    Lane 1: RAW264.7 cell lysate
    Lane 2: C2C12 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human UBE4B Polyclonal Antibody

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