Anti-Human UHRF1 Polyclonal Antibody (HP185024)

Name: Anti-Human UHRF1 Polyclonal Antibody
Brand: abinScience
SKU: HP185024
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP185024

Description

Anti-Human UHRF1 Polyclonal Antibody (HP185024) is a rabbit polyclonal antibody detecting UHRF1 in ELISA, IHC, WB. Suitable for Bovine, Danio rerio, Human, Xenopus laevis, and Xenopus tropicalis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human UHRF1 (Gly427-Gly636).

Target

Nuclear zinc finger protein Np95, RING-type E3 ubiquitin transferase UHRF1, RING finger protein 106, Transcription factor ICBP90, E3 ubiquitin-protein ligase UHRF1, Ubiquitin-like PHD and RING finger domain-containing protein 1, NP95, HuNp95, Nuclear protein 95, RNF106, ICBP90, hNp95, UHRF1, Inverted CCAAT box-binding protein of 90 kDa, hUHRF1, Ubiquitin-like-containing PHD and RING finger domains protein 1

Purification

Purified by antigen affinity column.

Accession

Q96T88

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase UHRF1 is a ~89 kDa protein. E3 ubiquitin-protein ligase that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Plays a key role in DNA methylation inheritance by promoting recruitment of DNMT1 to hemimethylated DNA and ensure faithful propagation of the DNA methylation patterns through DNA replication. Acts both as a histone reader and writer: specifically recognizes and binds (1) hemimethylated DNA at replication forks and (2) histone H3 trimethylated at 'Lys-9' and unmethylated at 'Arg-2' (H3K9me3 and H3R2me0, respectively), thereby activating its E3 ubiquitin-protein ligase activity. UHRF1 then mediates histone H3 'Lys-18' monoubiquitination (H3K18ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance. Also mediates histone H3 'Lys-14' and 'Lys-23' ubiquitination (H3K14ub and H3K23ub) at lower level.

1. Hopfner, R. et al. (2000) Cancer research 60, 121-8. PMID: 10646863

2. Arima, Y. et al. (2004) Genes to cells : devoted to molecular & cellular mechanisms 9, 131-42. PMID: 15009091

3. Kim, JK. et al. (2009) Nucleic acids research 37, 493-505. PMID: 19056828

4. Rothbart, SB. et al. (2012) Nature structural & molecular biology 19, 1155-60. PMID: 23022729

5. Nishiyama, A. et al. (2013) Nature 502, 249-53. PMID: 24013172

6. Harrison, JS. et al. (2016) eLife 5. PMID: 27595565

7. Vaughan, RM. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 8775-8780. PMID: 30104358

8. Foster, BM. et al. (2018) Molecular cell 72, 739-752.e9. PMID: 30392929

9. DaRosa, PA. et al. (2018) Molecular cell 72, 753-765.e6. PMID: 30392931

10. Tiedemann, RL. et al. (2024) Nucleic acids research 52, 13733-13756. PMID: 39607687

Note

For research use only.

Images

References

Datasheet

Anti-Human UHRF1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human UHRF1 Polyclonal Antibody (HP185024)

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Datasheet

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