Anti-Human UNG Polyclonal Antibody (HY404014)

Name: Anti-Human UNG Polyclonal Antibody
Brand: abinScience
SKU: HY404014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY404014

Description

Anti-Human UNG Polyclonal Antibody (HY404014) is a rabbit polyclonal antibody detecting UNG. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human UNG (Ala45-Leu313).

Target

DGU, EC:3.2.2.27, UDG, UNG, UNG1, UNG15, Uracil-DNA glycosylase

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P13051

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Uracil-DNA glycosylase (UNG) is a ~34 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication.

1. Imai, K. et al. (2003) Nature immunology 4, 1023-8. PMID: 12958596

2. Kavli, B. et al. (2005) The Journal of experimental medicine 201, 2011-21. PMID: 15967827

3. Akbari, M. et al. (2007) Neuroscience 145, 1201-12. PMID: 17101234

4. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144

5. Mol, CD. et al. (1995) Cell 82, 701-8. PMID: 7671300

6. Slupphaug, G. et al. (1996) Nature 384, 87-92. PMID: 8900285

7. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624

8. Bharati, S. et al. (1998) Nucleic acids research 26, 4953-9. PMID: 9776759

Note

For research use only

Images

References

Datasheet

Anti-Human UNG Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human UNG Polyclonal Antibody (HY404014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us