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Anti-Human UROD Polyclonal Antibody (HY326014)

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Overview
Catalog No.HY326014
Description
Anti-Human UROD Polyclonal Antibody (HY326014) is a rabbit polyclonal antibody detecting UROD in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human UROD (Met1-Asn367).
Target Uroporphyrinogen decarboxylase, UPD, URO-D, 4.1.1.37, UROD
Purification Purified by antigen affinity column.
Accession P06132
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Uroporphyrinogen decarboxylase (UROD) is a ~40 kDa protein. Catalyzes the sequential decarboxylation of the four acetate side chains of uroporphyrinogen to form coproporphyrinogen and participates in the fifth step in the heme biosynthetic pathway. Isomer I or isomer III of uroporphyrinogen may serve as substrate, but only coproporphyrinogen III can ultimately be converted to heme. In vitro also decarboxylates pentacarboxylate porphyrinogen I.

1. Brady, JJ. et al. (2000) The Journal of investigative dermatology 115, 868-74. PMID: 11069625
2. Phillips, JD. et al. (2001) Blood 98, 3179-85. PMID: 11719352
3. Phillips, JD. et al. (2003) The EMBO journal 22, 6225-33. PMID: 14633982
4. Cunha, L. et al. (2008) Proteins 71, 855-73. PMID: 18004775
5. To-Figueras, J. et al. (2011) The British journal of dermatology 165, 499-505. PMID: 21668429
6. Ged, C. et al. (2002) Archives of dermatology 138, 957-60. PMID: 12071824
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human UROD Polyclonal Antibody

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