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Anti-Human UROS Polyclonal Antibody (HY300014)

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Overview
Catalog No.HY300014
Description
Anti-Human UROS Polyclonal Antibody (HY300014) is a rabbit polyclonal antibody detecting UROS in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human UROS (Met1-Cys265).
Target Uroporphyrinogen-III synthase, UROIIIS, UROS, 4.2.1.75, Hydroxymethylbilane hydrolyase [cyclizing], Uroporphyrinogen-III cosynthase, UROS
Purification Purified by antigen affinity column.
Accession P10746
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Uroporphyrinogen-III synthase (UROS) is a ~28 kDa protein. Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrins. Porphyrins act as cofactors for a multitude of enzymes that perform a variety of processes within the cell such as methionine synthesis (vitamin B12) or oxygen transport (heme).

1. Mathews, MA. et al. (2001) The EMBO journal 20, 5832-9. PMID: 11689424
2. Cunha, L. et al. (2008) Proteins 71, 855-73. PMID: 18004775
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human UROS Polyclonal Antibody

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