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Anti-Human USP1 Polyclonal Antibody (HF660014)

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Overview
Catalog No.HF660014
Description
Anti-Human USP1 Polyclonal Antibody (HF660014) is a rabbit polyclonal antibody detecting USP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human USP1 (Arg486-Lys599).
Target Ubiquitin thioesterase 1, Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1, Ubiquitin-specific-processing protease 1, USP1, hUBP
Purification Purified by antigen affinity column.
Accession O94782
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ubiquitin carboxyl-terminal hydrolase 1 (USP1) is a ~88 kDa protein. Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.

1. Nijman, SM. et al. (2005) Molecular cell 17, 331-9. PMID: 15694335
2. Huang, TT. et al. (2006) Nature cell biology 8, 339-47. PMID: 16531995
3. Lee, KY. et al. (2010) The Journal of biological chemistry 285, 10362-9. PMID: 20147293
4. Cohn, MA. et al. (2007) Molecular cell 28, 786-97. PMID: 18082604
5. Yin, J. et al. (2015) Structure (London, England : 1993) 23, 2043-54. PMID: 26388029
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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