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Anti-Human USP21 Polyclonal Antibody (HP890014)

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Overview
Catalog No.HP890014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human USP21 (Thr220-Leu565).
Target Ubiquitin carboxyl-terminal hydrolase 21, Ubiquitin thioesterase 21, Deubiquitinating enzyme 21, USP21, Ubiquitin-specific-processing protease 21, USP23
Purification Purified by antigen affinity column.
Accession Q9UK80
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ubiquitin carboxyl-terminal hydrolase 21 (USP21) is a ~62 kDa protein. Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Deubiquitinates BAZ2A/TIP5 leading to its stabilization. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates.

1. Khan, A. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8338-43. PMID: 26100909
2. Gong, L. et al. (2000) The Journal of biological chemistry 275, 14212-6. PMID: 10799498
3. Garshott, DM. et al. (2020) eLife 9. PMID: 32011234
Note For research use only.
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Formula
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Anti-Human USP21 Polyclonal Antibody

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