Anti-Human USP22 Polyclonal Antibody (HP083014)

Name: Anti-Human USP22 Polyclonal Antibody
Brand: abinScience
SKU: HP083014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP083014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human USP22 (Gly182-Glu525).

Target

Deubiquitinating enzyme 22, USP22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, USP3L, Ubiquitin-specific-processing protease 22

Purification

Purified by antigen affinity column.

Accession

Q9UPT9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin carboxyl-terminal hydrolase 22 (USP22) is a ~59 kDa protein. Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. As part of the transcription regulatory histone acetylation (HAT) complex SAGA, catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a transcriptional coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Facilitates cell-cycle progression by stabilizing CCNB1 and antagonizing its proteasome-mediated degradation in a cell cycle-specific manner. Modulates cell cycle progression and apoptosis also by antagonizing TP53 transcriptional activation through deacetylase SIRT1 stabilization.

1. Zhao, Y. et al. (2008) Molecular cell 29, 92-101. PMID: 18206972

2. Zhang, XY. et al. (2008) Molecular cell 29, 102-11. PMID: 18206973

3. Zhang, XY. et al. (2008) Cell cycle (Georgetown, Tex.) 7, 1522-4. PMID: 18469533

4. Lin, Z. et al. (2015) Cell discovery 1, 15028-. PMID: 27030811

5. Lin, Z. et al. (2012) Molecular cell 46, 484-94. PMID: 22542455

6. Cai, Z. et al. (2020) The Journal of experimental medicine 217. PMID: 32130408

7. Karlowitz, R. et al. (2022) Cell death & disease 13, 684. PMID: 35933402

8. Huang, X. et al. (2019) Cancer immunology research 7, 1580-1590. PMID: 31399419

9. Roedig, J. et al. (2021) EMBO reports 22, e50163. PMID: 33369872

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-USP22 polyclonal antibody at 1µg/ml on various samples.
Lane 1: HepG2 cell lysate

References

Datasheet

Anti-Human USP22 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human USP22 Polyclonal Antibody (HP083014)

Overview

Images

References

Datasheet

SDS

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