Anti-Human USP3 Polyclonal Antibody (HP965014)

Name: Anti-Human USP3 Polyclonal Antibody
Brand: abinScience
SKU: HP965014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP965014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human USP3 (Gly316-Leu520).

Target

Deubiquitinating enzyme 3, Ubiquitin thioesterase 3, USP3, Ubiquitin-specific-processing protease 3, Ubiquitin carboxyl-terminal hydrolase 3

Purification

Purified by antigen affinity column.

Accession

Q9Y6I4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin carboxyl-terminal hydrolase 3 (USP3) is a ~58 kDa protein. Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. In response to DNA damage, deubiquitinates monoubiquitinated target proteins such as histone H2A and H2AX and thereby counteracts RNF168- and RNF8-mediated ubiquitination. In turn, participates in the recruitment of DNA damage repair factors to DNA break sites. Required for proper progression through S phase and subsequent mitotic entry. Acts as a positive regulator of TP53 by deubiquitinating and stabilizing it to promote normal cell proliferation and transformation.

1. Sharma, N. et al. (2014) Cell cycle (Georgetown, Tex.) 13, 106-14. PMID: 24196443

2. Nicassio, F. et al. (2007) Current biology : CB 17, 1972-7. PMID: 17980597

3. Fu, S. et al. (2017) Biochemical and biophysical research communications 492, 178-183. PMID: 28807825

4. Duan, T. et al. (2023) EMBO reports 24, e57828. PMID: 37971847

5. Cui, J. et al. (2014) Cell research 24, 400-16. PMID: 24366338

6. Zhuang, W. et al. (2022) Cellular & molecular immunology 19, 1141-1152. PMID: 36050480

Note

For research use only.

Images

References

Datasheet

Anti-Human USP3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human USP3 Polyclonal Antibody (HP965014)

Overview

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References

Datasheet

SDS

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