Anti-Human USP4 Polyclonal Antibody (HC280014)

Name: Anti-Human USP4 Polyclonal Antibody
Brand: abinScience
SKU: HC280014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC280014

Description

Anti-Human USP4 Polyclonal Antibody (HC280014) is a rabbit polyclonal antibody detecting USP4 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human USP4 (Asp12-Glu220).

Target

Ubiquitin carboxyl-terminal hydrolase 4, 3.4.19.12, Deubiquitinating enzyme 4, Ubiquitin thioesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog, USP4, UNP, UNPH

Endotoxin level

Please contact with the lab for this information.

Observed Molecular Weight

~110 kDa

Purification

Purified by antigen affinity column.

Accession

Q13107, A6QR55, B2GUZ1, P35123

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin carboxyl-terminal hydrolase 4 (USP4) is a ~108 kDa protein. Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. Deubiquitinates HAS2.

1. Wada, K. et al. (2006) Biochemical and biophysical research communications 339, 731-6. PMID: 16316627

2. Milojevic, T. et al. (2006) Molecular pharmacology 69, 1083-94. PMID: 16339847

3. Wada, K. et al. (2006) Biochemical and biophysical research communications 342, 253-8. PMID: 16472766

4. Song, EJ. et al. (2010) Genes & development 24, 1434-47. PMID: 20595234

5. Uras, IZ. et al. (2012) PloS one 7, e31003. PMID: 22347420

6. Clerici, M. et al. (2014) Nature communications 5, 5399. PMID: 25404403

7. Mehić, M. et al. (2017) Oncogenesis 6, e348. PMID: 28604766

8. Deng, L. et al. (2019) Cell research 29, 136-150. PMID: 30514904

9. Chu, F. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2401174121. PMID: 39589880

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-USP4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate

References

Datasheet

Anti-Human USP4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human USP4 Polyclonal Antibody (HC280014)

Overview

Images

References

Datasheet

SDS