Anti-Human VAPB Polyclonal Antibody (HF748014)

Name: Anti-Human VAPB Polyclonal Antibody
Brand: abinScience
SKU: HF748014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF748014

Description

Anti-Human VAPB Polyclonal Antibody (HF748014) is a rabbit polyclonal antibody detecting VAPB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human VAPB (Met1-Glu124).

Target

VAMP-B/VAMP-C, VAPB, VAP-B/VAP-C, Vesicle-associated membrane protein-associated protein B/C, VAMP-associated protein B/C

Purification

Purified by antigen affinity column.

Accession

O95292

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Vesicle-associated membrane protein-associated protein B/C (VAPB) is a ~27 kDa protein. Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. Interacts with STARD3 in a FFAT motif phosphorylation dependent manner. Via interaction with WDR44 participates in neosynthesized protein export. Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity. Involved in cellular calcium homeostasis regulation.

1. Lucken-Ardjomande Häsler, S. et al. (2020) The Journal of cell biology 219. PMID: 32344433

2. Di Mattia, T. et al. (2020) The EMBO journal 39, e104369. PMID: 33124732

3. Kanekura, K. et al. (2006) The Journal of biological chemistry 281, 30223-33. PMID: 16891305

4. Chen, HJ. et al. (2010) The Journal of biological chemistry 285, 40266-81. PMID: 20940299

5. De Vos, KJ. et al. (2012) Human molecular genetics 21, 1299-311. PMID: 22131369

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-VAPB polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: Mouse muscle lysate
Lane 4: Mouse brain lysate
Lane 5: Mouse liver lysate
Lane 6: Mouse lung lysate
Lane 7: Rat muscle lysate
Lane 8: Rat brain lysate
Lane 9: Rat liver lysate
Lane 10: Rat lung lysate

References

Datasheet

Anti-Human VAPB Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human VAPB Polyclonal Antibody (HF748014)

Overview

Images

References

Datasheet

SDS

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