Anti-Human VPS16 Polyclonal Antibody (HP969014)

Name: Anti-Human VPS16 Polyclonal Antibody
Brand: abinScience
SKU: HP969014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP969014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human VPS16 (Lys492-Lys839).

Target

hVPS16, VPS16, Vacuolar protein sorting-associated protein 16 homolog

Purification

Purified by antigen affinity column.

Accession

Q9H269

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Vacuolar protein sorting-associated protein 16 homolog (VPS16) is a ~94 kDa protein. Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for recruitment of VPS33A to the HOPS complex.

1. Kim, BY. et al. (2001) The Journal of biological chemistry 276, 29393-402. PMID: 11382755

2. Solinger, JA. et al. (2013) The FEBS journal 280, 2743-57. PMID: 23351085

3. Jiang, P. et al. (2014) Molecular biology of the cell 25, 1327-37. PMID: 24554770

4. Perini, ED. et al. (2014) Traffic (Copenhagen, Denmark) 15, 1366-89. PMID: 25266290

5. Wartosch, L. et al. (2015) Traffic (Copenhagen, Denmark) 16, 727-42. PMID: 25783203

6. Graham, SC. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 13345-50. PMID: 23901104

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-VPS16 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate
Lane 2: MOLT-4 cell lysate

References

Datasheet

Anti-Human VPS16 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human VPS16 Polyclonal Antibody (HP969014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us