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Anti-Human VPS33B Polyclonal Antibody (HP956014)

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Overview
Catalog No.HP956014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human VPS33B (Met1-Ala617).
Target Vacuolar protein sorting-associated protein 33B, hVPS33B, VPS33B
Purification Purified by antigen affinity column.
Accession Q9H267
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Vacuolar protein sorting-associated protein 33B (VPS33B) is a ~70 kDa protein. May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Required for proper trafficking and targeting of the collagen-modifying enzyme lysyl hydroxylase 3 (LH3) to intracellular collagen. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity.

1. Gruber, R. et al. (2017) The Journal of investigative dermatology 137, 845-854. PMID: 28017832
2. Bach, H. et al. (2008) Cell host & microbe 3, 316-22. PMID: 18474358
3. Cullinane, AR. et al. (2010) Nature genetics 42, 303-12. PMID: 20190753
Note For research use only.
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Formula
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Formula
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V₁ (Stock Vol.)
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Anti-Human VPS33B Polyclonal Antibody

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