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Anti-Human WDR18 Polyclonal Antibody (HP436014)

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Overview
Catalog No.HP436014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human WDR18 (Met1-Ala321).
Target WD repeat-containing protein 18, WDR18
Purification Purified by antigen affinity column.
Accession Q9BV38
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

WD repeat-containing protein 18 (WDR18) is a ~47 kDa protein. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit. May play a role during development.

1. Fanis, P. et al. (2012) Molecular & cellular proteomics : MCP 11, 1263-73. PMID: 22872859
2. Finkbeiner, E. et al. (2011) The EMBO journal 30, 1067-78. PMID: 21326211
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Anti-Human WDR18 Polyclonal Antibody

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