Anti-Human WDR4 Polyclonal Antibody (HD097014)

Name: Anti-Human WDR4 Polyclonal Antibody
Brand: abinScience
SKU: HD097014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HD097014

Description

Anti-Human WDR4 Polyclonal Antibody (HD097014) is a rabbit polyclonal antibody detecting WDR4. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WDR4 (Met1-Cys412).

Target

Protein Wuho homolog, WD repeat-containing protein 4, WDR4, hWH, tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P57081

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 is a ~45 kDa protein. Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs.

1. Alexandrov, A. et al. (2002) RNA (New York, N.Y.) 8, 1253-66. PMID: 12403464

2. Pandolfini, L. et al. (2019) Molecular cell 74, 1278-1290.e9. PMID: 31031083

3. Zhang, LS. et al. (2019) Molecular cell 74, 1304-1316.e8. PMID: 31031084

4. Ruiz-Arroyo, VM. et al. (2023) Nature 613, 383-390. PMID: 36599982

5. Li, J. et al. (2023) Nature 613, 391-397. PMID: 36599985

6. Jin, X. et al. (2023) Cell discovery 9, 65. PMID: 37369656

7. Dai, Z. et al. (2021) Molecular cell 81, 3339-3355.e8. PMID: 34352206

8. Orellana, EA. et al. (2021) Molecular cell 81, 3323-3338.e14. PMID: 34352207

9. Zhao, Z. et al. (2023) Cell 186, 3208-3226.e27. PMID: 37379838

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-WDR4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse kidney lysate
Lane 2: Rat kidney lysate

References

Datasheet

Anti-Human WDR4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human WDR4 Polyclonal Antibody (HD097014)

Overview

Images

References

Datasheet

SDS

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