Anti-Human WDR45 Polyclonal Antibody (HP297014)

Name: Anti-Human WDR45 Polyclonal Antibody
Brand: abinScience
SKU: HP297014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HP297014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WDR45 (Cys23-Ala184).

Target

WD repeat-containing protein 45, WD repeat domain phosphoinositide-interacting protein 4, WDR45, WDRX1, WDRXI4, WIPI-4, WIPI4

Purification

Purified by antigen affinity column.

Accession

Q9Y484

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is a ~39 kDa protein. Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Binds phosphatidylinositol 3-phosphate (PtdIns3P). Activated by the STK11/AMPK signaling pathway upon starvation, WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes. Together with WIPI1, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes. Probably recruited to membranes through its PtdIns3P activity.

1. Saitsu, H. et al. (2013) Nature genetics 45, 445-9, 449e1. PMID: 23435086

2. Bakula, D. et al. (2017) Nature communications 8, 15637. PMID: 28561066

3. Maeda, S. et al. (2019) eLife 8. PMID: 31271352

Note

For research use only.

Images

References

Datasheet

Anti-Human WDR45 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human WDR45 Polyclonal Antibody (HP297014)

Overview

Images

References

Datasheet

SDS

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