Anti-Human WEE1 Polyclonal Antibody (HB216014)

Name: Anti-Human WEE1 Polyclonal Antibody
Brand: abinScience
SKU: HB216014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HB216014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human WEE1 (Asn290-Ser573).

Target

Wee1A kinase, WEE1, Wee1-like protein kinase, WEE1hu

Purification

Purified by antigen affinity column.

Accession

P30291

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Wee1-like protein kinase is a ~71 kDa protein. Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

1. Watanabe, N. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 4419-24. PMID: 15070733

2. Watanabe, N. et al. (1995) The EMBO journal 14, 1878-91. PMID: 7743995

3. Heald, R. et al. (1993) Cell 74, 463-74. PMID: 8348613

4. McGowan, CH. et al. (1993) The EMBO journal 12, 75-85. PMID: 8428596

Note

For research use only.

Images

References

Datasheet

Anti-Human WEE1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human WEE1 Polyclonal Antibody (HB216014)

Overview

Images

References

Datasheet

SDS

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