Anti-Human WRAP53 Polyclonal Antibody (HA912014)

Name: Anti-Human WRAP53 Polyclonal Antibody
Brand: abinScience
SKU: HA912014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HA912014

Description

Anti-Human WRAP53 Polyclonal Antibody (HA912014) is a rabbit polyclonal antibody detecting WRAP53 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WRAP53 (Ser165-Tyr418).

Target

WD repeat-containing protein 79, TCAB1, WD40 repeat-containing protein antisense to TP53 gene, Telomerase Cajal body protein 1, WRAP53, WRAP53beta, WDR79

Purification

Purified by antigen affinity column.

Accession

Q9BUR4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Telomerase Cajal body protein 1 (WRAP53) is a ~59 kDa protein. RNA chaperone that plays a key role in telomere maintenance and RNA localization to Cajal bodies. Specifically recognizes and binds the Cajal body box (CAB box) present in both small Cajal body RNAs (scaRNAs) and telomerase RNA template component (TERC). Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, required to stimulate the catalytic activity of the complex. Acts by specifically binding the CAB box of the TERC RNA and controlling the folding of the CR4/CR5 region of the TERC RNA, a critical step for telomerase activity.

1. Nguyen, THD. et al. (2018) Nature 557, 190-195. PMID: 29695869

2. Chen, L. et al. (2018) Cell 174, 218-230.e13. PMID: 29804836

3. Tycowski, KT. et al. (2009) Molecular cell 34, 47-57. PMID: 19285445

4. Egan, ED. et al. (2010) Molecular and cellular biology 30, 2775-86. PMID: 20351177

5. Venteicher, AS. et al. (2009) Science (New York, N.Y.) 323, 644-8. PMID: 19179534

6. Vogan, JM. et al. (2015) The Journal of biological chemistry 290, 21320-35. PMID: 26170453

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human WRAP53 polyclonal antibody at 1ug/mL on recombinant Human WRAP53 (Catalog No. HA912012).

References

Datasheet

Anti-Human WRAP53 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human WRAP53 Polyclonal Antibody (HA912014)

Overview

Images

References

Datasheet

SDS

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