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Anti-Human YEATS4 Polyclonal Antibody (HF502014)

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Overview
Catalog No.HF502014
Description
Anti-Human YEATS4 Polyclonal Antibody (HF502014) is a rabbit polyclonal antibody detecting YEATS4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human YEATS4 (Met1-Ile227).
Target Gas41, Glioma-amplified sequence 41, YEATS4, GAS41, NuBI1, NuBI-1, YEATS domain-containing protein 4, NuMA-binding protein 1
Purification Purified by antigen affinity column.
Accession O95619
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

YEATS domain-containing protein 4 (YEATS4) is a ~26 kDa protein. Chromatin reader component of the NuA4 histone acetyltransferase (HAT) complex, a complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 diacetylated at 'Lys-18' and 'Lys-27' (H3K18ac and H3K27ac) or histone H3 diacetylated at 'Lys-14' and 'Lys-27' (H3K14ac and H3K27ac). Also able to recognize and bind crotonylated histone H3. May also recognize and bind histone H3 succinylated at 'Lys-122' (H3K122succ); additional evidences are however required to confirm this result in vivo. Plays a key role in histone variant H2AZ1/H2A.Z deposition into specific chromatin regions: recognizes and binds H3K14ac and H3K27ac on the promoters of actively transcribed genes and recruits NuA4-related complex to deposit H2AZ1/H2A.Z.

1. Cai, Y. et al. (2003) The Journal of biological chemistry 278, 42733-6. PMID: 12963728
2. Doyon, Y. et al. (2004) Molecular and cellular biology 24, 1884-96. PMID: 14966270
3. Hsu, CC. et al. (2018) Genes & development 32, 58-69. PMID: 29437725
4. Hsu, CC. et al. (2018) Cell discovery 4, 28. PMID: 29900004
5. Cho, HJ. et al. (2018) ACS chemical biology 13, 2739-2746. PMID: 30071723
6. Wang, Y. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 2365-2370. PMID: 29463709
Note For research use only.
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Datasheet
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Formula
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Anti-Human YEATS4 Polyclonal Antibody

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