Anti-Human YTHDC2 Polyclonal Antibody (HP001014)

Name: Anti-Human YTHDC2 Polyclonal Antibody
Brand: abinScience
SKU: HP001014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HP001014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human YTHDC2 (Glu185-Gly361).

Target

YTHDC2, hYTHDC2, YTH domain-containing protein 2, 3'-5' RNA helicase YTHDC2

Purification

Purified by antigen affinity column.

Accession

Q9H6S0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

3'-5' RNA helicase YTHDC2 is a ~160 kDa protein. 3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells. Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability. Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs. Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease. Required for both spermatogenesis and oogenesis.

1. Xu, C. et al. (2015) The Journal of biological chemistry 290, 24902-13. PMID: 26318451

2. Wojtas, MN. et al. (2017) Molecular cell 68, 374-387.e12. PMID: 29033321

3. Kretschmer, J. et al. (2018) RNA (New York, N.Y.) 24, 1339-1350. PMID: 29970596

Note

For research use only.

Images

References

Datasheet

Anti-Human YTHDC2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-Human YTHDC2 Polyclonal Antibody (HP001014)

Overview

Images

References

Datasheet

SDS

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