Anti-Human YWHAH Polyclonal Antibody (HC326014)

Name: Anti-Human YWHAH Polyclonal Antibody
Brand: abinScience
SKU: HC326014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC326014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human YWHAH (Met1-Asn246).

Target

YWHAH, Protein AS1, YWHA1, 14-3-3 protein eta

Purification

Purified by antigen affinity column.

Accession

Q04917

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

14-3-3 protein eta (YWHAH) is a ~28 kDa protein. Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-YWHAH polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: A431 cell lysate
Lane 3: Raji cell lysate
Lane 4: Hela cell lysate
Lane 5: Jurkat cell lysate
Lane 6: Mouse brain lysate
Lane 7: Mouse spleen lysate
Lane 8: Mouse kidney lysate
Lane 9: Mouse heart lysate
Lane 10: Rat brain lysate
Lane11: Rat spleen lysate
Lane12: Rat kidney lysate
Lane13: Rat heart lysate

References

Datasheet

Anti-Human YWHAH Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human YWHAH Polyclonal Antibody (HC326014)

Overview

Images

References

Datasheet

SDS

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