Anti-Human ZMYND8 Polyclonal Antibody (HP036014)

Name: Anti-Human ZMYND8 Polyclonal Antibody
Brand: abinScience
SKU: HP036014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP036014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ZMYND8 (His111-Thr388).

Target

Protein kinase C-binding protein 1, PRKCBP1, RACK7, ZMYND8, CTCL-associated antigen se14-3, Cutaneous T-cell lymphoma-associated antigen se14-3, Rack7, KIAA1125, Zinc finger MYND domain-containing protein 8

Purification

Purified by antigen affinity column.

Accession

Q9ULU4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

MYND-type zinc finger-containing chromatin reader ZMYND8 is a ~131 kDa protein. Chromatin reader that recognizes dual histone modifications such as histone H3.1 dimethylated at 'Lys-36' and histone H4 acetylated at 'Lys-16' (H3.1K36me2-H4K16ac) and histone H3 methylated at 'Lys-4' and histone H4 acetylated at 'Lys-14' (H3K4me1-H3K14ac). May act as a transcriptional corepressor for KDM5D by recognizing the dual histone signature H3K4me1-H3K14ac. May also act as a transcriptional corepressor for KDM5C and EZH2. Recognizes acetylated histone H4 and recruits the NuRD chromatin remodeling complex to damaged chromatin for transcriptional repression and double-strand break repair by homologous recombination. Also activates transcription elongation by RNA polymerase II through recruiting the P-TEFb complex to target promoters.

1. Adhikary, S. et al. (2016) The Journal of biological chemistry 291, 2664-81. PMID: 26655721

2. Li, N. et al. (2016) Molecular cell 63, 470-84. PMID: 27477906

3. Mukherjee, S. et al. (2020) Journal of biosciences 45. PMID: 31965980

4. Adhikary, S. et al. (2022) Cell death & disease 13, 766. PMID: 36064715

5. Mukherjee, S. et al. (2020) Cell death & disease 11, 1073. PMID: 33323928

6. Gong, F. et al. (2015) Genes & development 29, 197-211. PMID: 25593309

7. Spruijt, CG. et al. (2016) Cell reports 17, 783-798. PMID: 27732854

8. Ghosh, K. et al. (2018) Cell reports 24, 2141-2154.e6. PMID: 30134174

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human ZMYND8 polyclonal antibody at 1ug/mL on recombinant Human ZMYND8 (Catalog No. HP036012).

References

Datasheet