Anti-Human ZWINT Polyclonal Antibody (HF907014)

Name: Anti-Human ZWINT Polyclonal Antibody
Brand: abinScience
SKU: HF907014
Price: 96 USD
Availability: InStock

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SDS

Overview

Catalog No.

HF907014

Description

Anti-Human ZWINT Polyclonal Antibody (HF907014) is a rabbit polyclonal antibody detecting ZWINT in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ZWINT (Met1-Pro277).

Target

ZWINT

Purification

Purified by antigen affinity column.

Accession

O95229

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT

Background

Outer kinetochore KNL1 complex subunit ZWINT is a ~31 kDa protein. Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Targets the RZZ complex to the kinetochore at prometaphase. Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization.

1. Musio, A. et al. (2004) Gene 331, 33-40. PMID: 15094189

2. Wang, H. et al. (2004) The Journal of biological chemistry 279, 54590-8. PMID: 15485811

3. Kops, GJ. et al. (2005) The Journal of cell biology 169, 49-60. PMID: 15824131

4. Lin, YT. et al. (2006) Oncogene 25, 6901-14. PMID: 16732327

5. Petrovic, A. et al. (2014) Molecular cell 53, 591-605. PMID: 24530301

6. Petrovic, A. et al. (2016) Cell 167, 1028-1040.e15. PMID: 27881301

7. Yatskevich, S. et al. (2024) Nature structural & molecular biology 31, 874-883. PMID: 38459127

8. Polley, S. et al. (2024) Nature structural & molecular biology 31, 861-873. PMID: 38459128

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ZWINT polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: SW620 cell lysate
Lane 3: 293T cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Hela cell lysate
Lane 6: Raji cell lysate
Lane 7: K562 cell lysate

References

Datasheet

Anti-Human ZWINT Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ZWINT Polyclonal Antibody (HF907014)

Overview

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References

Datasheet

SDS

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