Anti-Hypusine Recombinant Antibody (Hpu24) (YP587013)

Name: Anti-Hypusine Recombinant Antibody (Hpu24)
Brand: abinScience
SKU: YP587013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

YP587013

Species reactivity

General

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clone ID

Hpu24

Clonality

Monoclonal

Target

Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

PubChem: 65396

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

References

1. Shiba T,et al. Hypusine, a new amino acid occurring in bovine brain. Isolation and structural determination. Biochim Biophys Acta. 1971 Sep 21;244(3):523-31. [YP587013]
2. ark MH, et al. Hypusine, a polyamine-derived amino acid critical for eukaryotic translation. J Biol Chem. 2018 Nov 30;293(48):18710-18718. [YP587013]

Note

For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Hypusine antibody (Hpu24).
SDS-PAGE

SDS-PAGE for Anti-Hypusine antibody (Hpu24).
Western blot

Western blot analysis was performed using anti-EIF5A monoclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: Mouse pancears lysate
Lane 7: Rat pancears lysate
Western blot

Western blot analysis was performed using anti-Eif5a polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: K562 cell lysate
Lane 4: PC12 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: 293T cell lysate