Anti-IDE Polyclonal Antibody (HB619014)

Name: Anti-IDE Polyclonal Antibody
Brand: abinScience
SKU: HB619014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB619014

Description

Anti-IDE Polyclonal Antibody (HB619014) is a rabbit polyclonal antibody detecting IDE in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IDE (Ala753-Pro973).

Target

Insulysin, IDE, Abeta-degrading protease, Insulin protease, Insulinase, Insulin-degrading enzyme

Purification

Purified by antigen affinity column.

Accession

P14735

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Insulin-degrading enzyme (IDE) is a ~117 kDa protein. Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling. Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin. Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP. Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP.

1. Vekrellis, K. et al. (2000) The Journal of neuroscience : the official journal of the Society for Neuroscience 20, 1657-65. PMID: 10684867

2. Shen, Y. et al. (2006) Nature 443, 870-4. PMID: 17051221

3. Im, H. et al. (2007) The Journal of biological chemistry 282, 25453-63. PMID: 17613531

4. Malito, E. et al. (2008) Biochemistry 47, 12822-34. PMID: 18986166

5. Manolopoulou, M. et al. (2009) The Journal of biological chemistry 284, 14177-88. PMID: 19321446

6. Ralat, LA. et al. (2011) The Journal of biological chemistry 286, 4670-9. PMID: 21098034

7. Affholter, JA. et al. (1990) Molecular endocrinology (Baltimore, Md.) 4, 1125-35. PMID: 2293021

8. McCord, LA. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 13827-32. PMID: 23922390

9. Maianti, JP. et al. (2014) Nature 511, 94-8. PMID: 24847884

10. Deprez-Poulain, R. et al. (2015) Nature communications 6, 8250. PMID: 26394692

Note

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting IDE (HB619014) at a concentration of 12.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of IDE was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet